Results Validation of Transfection of GSTP1 in HepG2 Cells To evaluate the role of GSTP1 in protecting against QIs-induced cellular perturbations, HepG2 cells were transiently transfected with a DNA construct containing the human GSTP1 cDNA behind a CMV-promoter
Interestingly, homocysteine levels have been positively associated with serum GGT, suggesting elevations in homocysteine may at least partly be due to increased shunting toward glutathione synthesis in response to increased GSH demands, though this has not been proven as of yet
1 In the USA, over 67% of adults report daily coffee consumption, averaging more than 1.5 cups per day (~135 mg), with 36% of adults reporting an average daily consumption of 3-5 cups
Furthermore, AQ-QI- and DEAQ-QI-induced cytotoxicity was reduced in GSTP1 -transfected cells under GSH-depleted conditions
This observation lacks rigorous controlled trial data but appears consistently in user reports
The scientific data and practical issues for individuals interested in glutathione injections as a wound healing therapy are examined in this article
